So you see, the response requested is indicated in the very form and name of the document itself. The Letter of Intent has no established place in International trade law. Where they are used _ rarely _ is in some small niche internal markets and trades. The RFQ, Request for Quotation, a standard protocol in the business world, is what most brokers actually want when they err in using an LOI.
At the end you should sign off with "sincerely yours" or any other related salutation. If you are writing a letter of intent for graduate school then it would have a different content. First of all letter of intent would start with your name, address, contact number and email address. After you have mentioned the date, you should then add a salutation like "Dear Sir/Mam".